Video explaining how sanger sequencing works.
How to cite Standard Sequencing Services in Materials and Methods:
DNA Sequencing of purified products (PCR or Plasmid) was carried out at the DNA Analysis Facility on Science Hill at Yale University (New Haven, CT). Reactions were preformed using BigDye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, ThermoFischer Scientific, Inc.) Dye terminator removal was performed using Performa® DTR Ultra 96-Well Plates (Edge Biosystems). Capillary electrophoresis was performed on a 3730xl DNA Analyzer (Applied Biosystems, Thermo Fischer Scientific, Inc.) with a 96-capillary 50cm array, using default instrument protocols.